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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/01/1998 |
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Data da última atualização: |
12/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TORRES, G. A.; PARENTONI, S. N.; LOPES, M. A.; PAIVA, E. |
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Afiliação: |
EMBRAPA/CNPMS; SIDNEY NETTO PARENTONI, CNPMS. |
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Título: |
A search for RFLP markers to identify genes for aluminum tolerance in maize. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Revista Brasileira de Genética, Ribeirão Preto, v. 20, n. 3, p. 459-465, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested for their ability to discriminate the parental lines. Fifty-four of these probes were polymorphic, with 46 showing codominance. These probes were hybridized with DNA from the two contrasting bulks. Three RFLPs on chromosome 8 distinguished the bulks on the basis of band intensity. DNA of individuals from the bulks was hybridized with these probes and showed the presence of heterozygous individuals in each bulk. These results suggest that in maize there is a region related to aliminum tolerance on chromosome 8. MenosThe objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested f... Mostrar Tudo |
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Palavras-Chave: |
Maize; RFLP; Tolerance; Tolerancia. |
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Thesagro: |
Alumínio; Milho; Zea Mays. |
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Thesaurus Nal: |
aluminum. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/43456/1/Search-RFLP.pdf
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Marc: |
LEADER 02698naa a2200253 a 4500
001 1478779
005 2018-06-12
008 1997 bl uuuu u00u1 u #d
100 1 $aTORRES, G. A.
245 $aA search for RFLP markers to identify genes for aluminum tolerance in maize.$h[electronic resource]
260 $c1997
520 $aThe objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested for their ability to discriminate the parental lines. Fifty-four of these probes were polymorphic, with 46 showing codominance. These probes were hybridized with DNA from the two contrasting bulks. Three RFLPs on chromosome 8 distinguished the bulks on the basis of band intensity. DNA of individuals from the bulks was hybridized with these probes and showed the presence of heterozygous individuals in each bulk. These results suggest that in maize there is a region related to aliminum tolerance on chromosome 8.
650 $aaluminum
650 $aAlumínio
650 $aMilho
650 $aZea Mays
653 $aMaize
653 $aRFLP
653 $aTolerance
653 $aTolerancia
700 1 $aPARENTONI, S. N.
700 1 $aLOPES, M. A.
700 1 $aPAIVA, E.
773 $tRevista Brasileira de Genética, Ribeirão Preto$gv. 20, n. 3, p. 459-465, 1997.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
27/08/2001 |
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Data da última atualização: |
17/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Nacional - B |
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Autoria: |
LOPES, P. M. O.; SILVA, B. B. da; AZEVEDO, P. V. de; SILVA, V. de P. R. da; TEIXEIRA, A. H. de C.; SOARES, J. M.; ESPINOLA SOBRINHO, J. |
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Afiliação: |
Pabrício Marcos Oliveira Lopes; Bernardo Barbosa da Silva; Pedro Vieira de Azevedo; Vicente de Paulo Rodrigues da Silva; ANTONIO HERIBERTO DE C TEIXEIRA, CPATSA; JOSÉ MONTEIRO SOARES, CPATSA; José Espínola Sobrinho. |
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Título: |
Balanco de energia num pomar de mangueiras irrigado. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Revista Brasileira de Agrometeorologia, Santa Maria, v. 9, n. 1, p. 1-8, 2001. |
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Idioma: |
Português |
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Conteúdo: |
Objetivando avaliar os valores diurnos dos componentes do balanco de energia, baseado na razao de Bowen, num pomar de mangueiras (Mangifera indica L), variedade Tommy Atkins, irrigado por gotejamento, foram analisados dados de um experimento agrometeorologico conduzido no periodo de 6 de agosto a 22 de novembro de 1998, no Projeto Bebedouro em Petrolina, PE (09"S; 4(;'22 'W;. 365,5m). Os resultados mostraram que o fluxo de calor no solo representa uma pequena fracao do saldo de radiacao, com valores em geral menores ou iguais a 7% do saldo de radiacao. Os padroes do saldo ide radiacao (Rn) e dos fluxos de calor latente (LE), de calor sensivel para o ar (H) e para o solo (G), foram semelhantes aqueles observados em culturas de pequeno e medio porte, apesar da grande variabilidade da nebulosidade. A temperatura diurna da copa das mangueiras foi; em geral, maior que a do ar em todos os estadios do ciclo produtivo do mangueiral, tendo sido registradas eventuais alternancias nos sinais do fluxo de calor sensivel. O fluxo de calor latente representou uma parcela significativa do saldo de radiacao, com valores superiores a 80% deste no estadio de queda fisiologica dos frutos. No final do ciclo produtivo, ocorreu uma sensivel reducao da evapotranspiracao do pomal: Observou-se, ainda, que o erro relativo associado a detenninacao do fluxo de calor latente variou de 5% a 30%. |
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Palavras-Chave: |
Fluxos de calor sensível e latente; Radiation; Saldo de radiação; Tommy Atkins; Variedade Tommy Atkins; Variety. |
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Thesagro: |
Balanço de Energia; Manga; Mangifera Indica; Radiação. |
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Thesaurus NAL: |
energy balance. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183114/1/Revista-Brasileira-de-Agrometeorologia-v.9-n.1-p.1-8-2001.pdf
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Marc: |
LEADER 02308naa a2200325 a 4500
001 1134336
005 2018-09-17
008 2001 bl uuuu u00u1 u #d
100 1 $aLOPES, P. M. O.
245 $aBalanco de energia num pomar de mangueiras irrigado.
260 $c2001
520 $aObjetivando avaliar os valores diurnos dos componentes do balanco de energia, baseado na razao de Bowen, num pomar de mangueiras (Mangifera indica L), variedade Tommy Atkins, irrigado por gotejamento, foram analisados dados de um experimento agrometeorologico conduzido no periodo de 6 de agosto a 22 de novembro de 1998, no Projeto Bebedouro em Petrolina, PE (09"S; 4(;'22 'W;. 365,5m). Os resultados mostraram que o fluxo de calor no solo representa uma pequena fracao do saldo de radiacao, com valores em geral menores ou iguais a 7% do saldo de radiacao. Os padroes do saldo ide radiacao (Rn) e dos fluxos de calor latente (LE), de calor sensivel para o ar (H) e para o solo (G), foram semelhantes aqueles observados em culturas de pequeno e medio porte, apesar da grande variabilidade da nebulosidade. A temperatura diurna da copa das mangueiras foi; em geral, maior que a do ar em todos os estadios do ciclo produtivo do mangueiral, tendo sido registradas eventuais alternancias nos sinais do fluxo de calor sensivel. O fluxo de calor latente representou uma parcela significativa do saldo de radiacao, com valores superiores a 80% deste no estadio de queda fisiologica dos frutos. No final do ciclo produtivo, ocorreu uma sensivel reducao da evapotranspiracao do pomal: Observou-se, ainda, que o erro relativo associado a detenninacao do fluxo de calor latente variou de 5% a 30%.
650 $aenergy balance
650 $aBalanço de Energia
650 $aManga
650 $aMangifera Indica
650 $aRadiação
653 $aFluxos de calor sensível e latente
653 $aRadiation
653 $aSaldo de radiação
653 $aTommy Atkins
653 $aVariedade Tommy Atkins
653 $aVariety
700 1 $aSILVA, B. B. da
700 1 $aAZEVEDO, P. V. de
700 1 $aSILVA, V. de P. R. da
700 1 $aTEIXEIRA, A. H. de C.
700 1 $aSOARES, J. M.
700 1 $aESPINOLA SOBRINHO, J.
773 $tRevista Brasileira de Agrometeorologia, Santa Maria$gv. 9, n. 1, p. 1-8, 2001.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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